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Comparing the Effects of black seed ethanol extract and chlorhexidine on Streptococcus mutans in laboratory conditions
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Hanieh Jadidi   , Seyyedabbas Alinezhadberijani , Peyvand Moeini , Sepideh Majzoubi *  |
| , se.majzoubi@gmail.com |
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Abstract: (193 Views) |
Abstract
Background and Aim: Streptococcus mutans is a key contributor to dental caries. Herbal compounds are increasingly explored as alternatives to chemical drugs with fewer side effects. This study compared the effect of ethanolic black seed extract and chlorhexidine on S. mutans under laboratory conditions.
Materials and Methods: Streptococcus mutans (PTCC1687) was obtained and the ethanolic black seed extract prepared by soaking in ethanol. The disk diffusion method on Mueller-Hinton agar was used to evaluate antibacterial activity at extract concentrations ranging from 0.09% to 50%. The diameter of inhibition zones was measured, and MIC/MBC values were determined using Microdilution and Macrodilution methods. Each experiment was repeated three times. Statistical analysis was performed using the T-test in SPSS software version 25.
Results: The mean inhibition zone for the black seed extract was 16 mm, whereas chlorhexidine produced a 20 mm zone. For black seed extract, the MIC was 12.5 mg/ml and the MBC was 25 mg/ml. For chlorhexidine, these values were 0.6 mg/ml (MIC) and 1.2 mg/ml (MBC). The T-test showed a significant difference (P < 0.05) in inhibition zone diameters between the black seed extract and chlorhexidine.
Conclusion: Ethanolic black seed extract displayed a positive inhibitory effect on S. mutans but was less effective than chlorhexidine. Further research may focus on optimizing black seed extract’s antimicrobial properties to provide a viable natural alternative in oral health applications.
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Article number: 2 |
| Keywords: Streptococcus mutans, Microbial Sensitivity Tests, Nigella sativa, chlorhexidine |
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Full-Text [PDF 745 kb]
(127 Downloads)
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Type of Study: original article |
Subject:
Pediatrics
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