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:: Volume 20, Issue 3 (jrds 2023) ::
J Res Dent Sci 2023, 20(3): 130-138 Back to browse issues page
The comparison of Licorice Root Ethanolic Extract, Chlorhexidine and Doxycycline Antibacterial effects on Porphyromonas gingivalis-An In Vitro Study
Mohammadreza Tabatabaeian , Vahid Esfahanian * , Arezoo Tahmourespour
Associate professor, Department of Periodontology, Faculty of dentistry, Isfahan(khorasgan) Branch, Islamic Azad university, Isfahan, Iran
Abstract:   (744 Views)
Background and Aims: Periodontal disease causes the destruction of tooth supporting tissue. Anaerobic Gram-negative bacterium, Porphyromonas gingivalis is one of the causes of this disease. Recently, due to the many side effects of mouthwashes and antibiotics, herbal materials have been receiving attention. licorice plant, with the scientific name Glycyrrhiza glabra, has antiviral, antibacterial and anti-inflammatory properties. Therefore, the purpose of this laboratory study is to investigate the antibacterial effect of different concentrations of licorice root ethanolic extract compared to doxycycline and chlorhexidine on Porphyromonas gingivalis bacteria.
Material and Methods: After preparing Porphyromonas gingivalis(ATCC 33277) bacteria from Isfahan University of Medical Sciences and bacterial culture and preparation of the plant and extraction, 6 dilutions of the extract were prepared. The test was carried out against Porphyromonas gingivalis bacteria along with 0.2% chlorhexidine mouthwash and doxycycline 100 mg antibiotic, with two methods of disk diffusion and microdilution. Data were analyzed by Mann-Whitney and Kruskal-Wallis statistical tests in spss26 software at a significance level of 5%.
Result: All six concentrations had significant antibacterial properties compared to each other and to chlorhexidine and doxycycline(P=0/001). The inhibitory concentration of extract, MIC70 and MIC90, was related to 6.25 and 200 mg/ml concentrations, respectively. The inhibitory percentages of chlorhexidine and doxycycline were 82.4% and 77.8%, respectively.
Conclusion: Licorice root ethanolic extract showed a very good antimicrobial effect. So that in higher concentrations, an equal or even greater effect was obtained than the chlorhexidine and doxycycline.
 
Article number: 7
Keywords: Antibacterial agents, Licorice, Chlorhexidine
Full-Text [PDF 794 kb]   (638 Downloads)    
Type of Study: original article | Subject: Perio
References
1. Dewake N, Ma X, Sato K, Nakatsu S, Yoshimura K, Eshita Y, et al. β-Glycyrrhetinic acid inhibits the bacterial growth and biofilm formation by supragingival plaque commensals. Microbiol Immunol. 2021 Sep;65(9):343-51. [DOI:10.1111/1348-0421.12884] [PMID]
2. Rezaie E, Bayani M, Arjomandzadegan M. The Inhibitory and Antibacterial Effects of Peppermint Essential Oil on Periodontal Photogenes. J Arak Univ Med Sci. 2020 Aug 30 [cited 2022 Jan 11];23(2):172-83. [DOI:10.32598/JAMS.23.2.5710.3]
3. Carranza F, Newman M. G, Takei H KD. Carranza's clinical periodontology.11 th ed.St Louis:Elsevier,2012:(12-26) (58-80) (84-103) (129-131) (281-282).
4. Binti Badlishah Sham NI, Lewin SD, Grant MM. Proteomic Investigations of In Vitro and In Vivo Models of Periodontal Disease. Proteomics Clin Appl. 2020 May;14(3):e1900043. [DOI:10.1002/prca.201900043] [PMID]
5. Mahmudpourmoteshakker T, Rafiee E, Farhad S AA. Comparison of the Effect of Doxycycline and Licorice on Chronic Periodontitis - A Clinical Trial Study. J Res Dent Sci. 2014;11(3):123-9.
6. Tanabe S, Desjardins J, Bergeron C, Gafner S, Villinski JR, Grenier D. Reduction of bacterial volatile sulfur compound production by licoricidin and licorisoflavan A from licorice. J Breath Res. 2012 Mar;6(1):16006. [DOI:10.1088/1752-7155/6/1/016006] [PMID]
7. La VD, Tanabe S, Bergeron C, Gafner S, Grenier D. Modulation of matrix metalloproteinase and cytokine production by licorice isolates licoricidin and licorisoflavan A: potential therapeutic approach for periodontitis. J Periodontol. 2011 Jan;82(1):122-8. [DOI:10.1902/jop.2010.100342] [PMID]
8. Lang NP, Berglundh T, Giannobile WV, Sanz M E. Lindhe's Clinical Periodontology and Implant Dentistry, 2 Volume Set. John Wiley & Sons; 2021 Oct 18.
9. Chitsazi M, Shirmohammadi A BE. اEffects of herbal mouthwash on periodontal indexes: comparable persica, Matrica, chlorhexidine. J Dent Shiraz Univ Med Sci. 2007;8(4 (17)):54-60.
10. Farhad SZ, Aminzadeh A, Mafi M, Barekatain M, Naghney M, Ghafari MR. The effect of adjunctive low-dose doxycycline and licorice therapy on gingival crevicular fluid matrix metalloproteinase-8 levels in chronic periodontitis. Dent Res J (Isfahan). 2013;10(5):624-9.
11. Kanwar I, Sah AK, Suresh PK. Biofilm-mediated Antibiotic-resistant Oral Bacterial Infections: Mechanism and Combat Strategies. Curr Pharm Des. 2017;23(14):2084-95. [DOI:10.2174/1381612822666161124154549] [PMID]
12. Khoshnam S E, Farzaneh M, valipour M, Bahaoddini A valipour A. Review of the phytochemical, pharmacological and physiological properties of Licorice (Glycyrrhizaglabra). Vol. 4, Clinical-Excellence. 2015. p. 56-71.
13. Mamedov NA, Egamberdieva D. Phytochemical Constituents and Pharmacological Effects of Licorice: A Review. In: Ozturk M, Hakeem KR, editors. Plant and Human Health, Volume 3: Pharmacology and Therapeutic Uses. Cham: Springer International Publishing; 2019. p. 1-21. [DOI:10.1007/978-3-030-04408-4_1] [PMCID]
14. Hasan MK, Ara I, Mondal MSA, Kabir Y. Phytochemistry, pharmacological activity, and potential health benefits of Gly cyrrhiza glabra. Heliyon. 2021 Jun;7(6):e07240. [DOI:10.1016/j.heliyon.2021.e07240] [PMID] [PMCID]
15. Azmoudeh F, Aslanimehr M, Lourizadeh N. Effect of Glycyrrhiza glabra extract on Streptococcus mutans and Candida albicans (in vitro study). URMIAMJ. 2017 Sep 1;28(6):394-400.
16. Khanahmadi M M, Naghdi Badi H, Akhondzadeh S, Khalighi Sigaroodi F, Mehrafarin A SS et al . A Review on Medicinal Plant of Glycyrrhiza glabra L. J Med Plants. 2013;12(46):1-12.
17. El-Saber Batiha G, Magdy Beshbishy A, El-Mleeh A, Abdel-Daim MM, Prasad Devkota H. Traditional Uses, Bioactive Chemical Constituents, and Pharmacological and Toxicological Activities of Glycyrrhiza glabra L. (Fabaceae). Biomolecules. 2020 Feb;10(3). [DOI:10.3390/biom10030352] [PMID] [PMCID]
18. Jiang M, Zhao S, Yang S, Lin X, He X, Wei X, et al. An "essential herbal medicine"-licorice: A review of phytochemicals and its effects in combination preparations. J Ethnopharmacol. 2020 Mar;249:112439. [DOI:10.1016/j.jep.2019.112439] [PMID]
19. Sidhu P, Shankargouda S, Rath A, Hesarghatta Ramamurthy P, Fernandes B, Kumar Singh A. Therapeutic benefits of liquorice in dentistry. J Ayurveda Integr Med. 2020;11(1):82-8. [DOI:10.1016/j.jaim.2017.12.004] [PMID] [PMCID]
20. Youngseok H, Tae-Jong K. Conditions for Preparing Glycyrrhiza uralensis Extract for Inhibiting Biofilm Formation of Streptococcus mutans. 2019 Mar 25;47(2):178-88. [DOI:10.5658/WOOD.2019.47.2.178]
21. Malvania EA, Sharma AS, Sheth SA, Rathod S, Chovatia NR, Kachwala MS. In Vitro Analysis of Licorice (Glycyrrhiza glabra) Root Extract Activity on Streptococcus mutans in Comparison to Chlorhexidine and Fluoride Mouthwash. J Contemp Dent Pract. 2019 Dec;20(12):1389-94. [DOI:10.5005/jp-journals-10024-2724] [PMID]
22. Haraszthy VI, Zambon JJ, Trevisan M, Zeid M GR. Identification of periodontal pathogens in atheromatous plaques. J Periodontol. 2000 Oct;71(10):1554-60. [DOI:10.1902/jop.2000.71.10.1554] [PMID]
23. Gunsolley JC. Clinical efficacy of antimicrobial mouthrinses. Journal of dentistry. 2010 Jun 1;38:S6-10. [DOI:10.1016/S0300-5712(10)70004-X] [PMID]
24. Fabricant DS, Farnsworth NR. The value of plants used in traditional medicine for drug discovery. Environmental health perspectives. 2001 Mar;109(suppl 1):69-75. [DOI:10.1289/ehp.01109s169] [PMID] [PMCID]
25. Burt S. Essential oils: their antibacterial properties and potential applications in foods-a review. International journal of food microbiology. 2004 Aug 1;94(3):223-53. [DOI:10.1016/j.ijfoodmicro.2004.03.022] [PMID]
26. Ajagannanavar SL, Battur H, Shamarao S, Sivakumar V, Patil PU, Shanavas P. Effect of aqueous and alcoholic licorice (glycyrrhiza glabra) root extract against streptococcus mutans and lactobacillus acidophilus in comparison to chlorhexidine: an in vitro study. J Int oral Heal JIOH. 2014;6(4):29-34. [DOI:10.4103/2231-0762.146215] [PMID] [PMCID]
27. Suwannakul S, Chaibenjawong P. Antibacterial Activities of Glycyrrhiza gabra Linn. (Licorice) Root Extract against Porphyromonas gingivalis rand Its Inhibitory Effects on Cysteine Proteases and Biofilms. J Dent Indones. 2017 Dec 31;24. [DOI:10.14693/jdi.v24i3.1075]
28. Villinski JR, Bergeron C, Cannistra JC, Gloer JB, Coleman CM, Ferreira D, et al. Pyrano-isoflavans from Glycyrrhiza uralensis with antibacterial activity against Streptococcus mutans and Porphyromonas gingivalis. J Nat Prod. 2014 Mar;77(3):521-6. [DOI:10.1021/np400788r] [PMID]
29. Wittschier N, Faller G, Hensel A. Aqueous extracts and polysaccharides from liquorice roots (Glycyrrhiza glabra L.) inhibit adhesion of Helicobacter pylori to human gastric mucosa. J Ethnopharmacol. 2009 Sep;125(2):218-23. [DOI:10.1016/j.jep.2009.07.009] [PMID]
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Tabatabaeian M, Esfahanian V, Tahmourespour A. The comparison of Licorice Root Ethanolic Extract, Chlorhexidine and Doxycycline Antibacterial effects on Porphyromonas gingivalis-An In Vitro Study. J Res Dent Sci 2023; 20 (3) : 7
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Volume 20, Issue 3 (jrds 2023) Back to browse issues page
مجله تحقیق در علوم دندانپزشکی Res Dent Sci
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