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:: Volume 7, Issue 4 (Res Dent Sci 2011) ::
J Res Dent Sci 2011, 7(4): 1-10 Back to browse issues page
Effect of Chemical and Mechanical Titanium Surface Treatment on fibroblast attachment and viability
Ali Rajaei * , Morteza Daliri joupari Dr
Islamic Azad University , rajaee.ali@gmail.com rajaee.ali@gmail.com
Abstract:   (21715 Views)

 

  Rajaei A #1 Daliri Joupari M 2

  1- MS in Biological Materials

  2- Assistant Professor of Biomedical Engineering Department, National Research Center for Genetic Engineering and Biotechnology, Tehran, Iran

  Corresponding Author : Rajaei A: MS in Biological Materials, No.14, west Vafadar Ave., Tehranpars. Tehran, Iran

  Tel: 09123266645 Email: rajaee.ali@gmail.com

  Background and Aim:

  Cell-titanium interactions are crucial to the clinical success of bone and dental implants. Mechanical or chemical surface treatment can help the cell attachment to implant surface. The purpose of this study was to investigate the effect of two distinct surface treatment( sandblasted and acid-etched) on fibroblasts cell attachment and viability.

  Materials and Methods:

  In this experimental study the fibroblasts behavior was analyzed on three different titanium surfaces: sand blasted(SB), Acid-Etched (AE)and pure commercial titanium(PCT) ,in three groups,(N=10). Scanning electron microscopy (SEM) showed distinct microtopographies. Cell morphology and initial attachment were evaluated by SEM . Cell viability was measured by means of a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The number of viable cell counts were compared using one way ANOVA Test.

  Results:

  SEM observation revealed drastic differences in surface microtopography, with a higher cell density on (SB)than (AE)and(PCT). Cell attachment were much better in the sand-blasted group than others. Cell viability recorded by the sand-blasted group( 1.28±0.58) was significantly better than acid-ethed (0.95±0.23) and pure commercial titanium(1.67±0.17).(P < 0.05)

  Conclusion:

  Implant surface treatments influence the attachment and viability of fibroblasts(L-929), and sand-blasted treatment seems to be the most favorable surface to compare with acid-etched and and pure commercial titanium surface.

  Keywords: Titanium implant, Sand-blasted , Acid-Etched, cell adhesion, , MTTviability assay, Fibroblast

 

Keywords: Titanium implant, Sand-blasted, Acid-Etched, cell adhesion, MTTviability assay, Fibroblast
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Type of Study: original article | Subject: oral surgery
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Rajaei A, Daliri joupari M. Effect of Chemical and Mechanical Titanium Surface Treatment on fibroblast attachment and viability. J Res Dent Sci. 2011; 7 (4) :1-10
URL: http://jrds.ir/article-1-29-en.html


Volume 7, Issue 4 (Res Dent Sci 2011) Back to browse issues page
مجله تحقیق در علوم دندانپزشکی Res Dent Sci
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