Background and Aim: Contaminated toothbrush has important role in transmission of infection and disease. The purpose of this study was to evaluate two methods of toothbrush disinfection by sodium hypochlorite 0/1% and microwave on three types of microorganisms: staphylococcus aureus, streptococcus mutans and candida albicans.
Materials and methods: In this in vitro study, 90 toothbrushes were divided into 9 groups (n=10). Toothbrushes were contaminated by standard suspension of streptococcus mutans, staphylococcus aureus and candida albicans. Then, toothbrushes were disinfected with hypochlorite sodium %0.1 for 10 minutes and with microwave for 1 minute. Afterwards, remained bacteria on surface of toothbrush were cultured and counted. For analyzing Numbers of Colony-Forming Units remained on toothbrush one-way Kruskal-Wallis and Mann-Whitney tests were used.
Results: Results of this study showed that significant difference exists among disinfectant effects (p<0.001). Results ofMann-Whitney test showedhypochlorite sodium had more disinfecting effect on Streptococcus mutans and Candida albicans compared with distilled water or microwave (p<0.05).However disinfecting effect of microwave was more than two other disinfectants on Staphylococcus aureus(p<0.05).
Conclusion: hypochlorite sodium had more disinfecting effect on Streptococcus mutans and Candida albicans however microwave was more effective on Staphylococcus aureus.
Behfarnia P, Talebi A, Samety A A, Mohammadinia A. Evaluating the Disinfecting Effect of Microwave and 0.1% Sodium Hypochlorite on Contaminated Toothbrushes. J Res Dent Sci 2014; 11 (3) :152-159 URL: http://jrds.ir/article-1-517-en.html