Background & aim: To ensure root canal treatment success, endodontic microbiota should be efficiently reduced. Several irrigation devices have been recently introduced with the main objective of improving root canal disinfection .The purpose of this study was to evaluate the rinsing effect of RinsEndo system in reduction of enterococcus faecalis in comparison with conventional hand syringe in infected root canals.

Method and Materials: In this experimental study,60 extracted single root and single canal anterior teeth were infected with enterococcus faecalis and divided into 3 groups: RinsEndo system, conventional hand syringe and control group. The enterococcus faecalis colonies were counted in each group before and after rinsing. Data was analyzed with parametric tests such as ANOVA and nonparametric test like KrusKal Wallis.

Results: The mean of enterococcus faecalis growth after rinsing was 3.50×10³ in group with conventional syring rinsing, 2.04×10³ in group with RinsEndo washing and 6.11×10³ in control group. Reduction of enterococcus faecalis after rinsing was statistically significant in each group (P<0.001). The amount of reduction in number of colonies with RinsEndo and conventional syringe rinsing was higher in comparison with control group and this difference was significant (P<0.001). RinsEndo rinsing effect was statistically significantly higher in comparison to conventional syringe as well (P<0.001).

Conclusions: Rinsing with RinsEndo system is significantly more efficient in reduction of enterococcus faecalis from root canal in comparison with hand syringe washing.