fa بررسی اثر ضد قارچی عصاره‌های آبی و الکلی پالپ و پوست موز (گروه Musa AAA، زیرگروه Cavendish) بر کاندیدا آلبیکنس استاندارد بیماری دهان Oral Medicine مطالعه اصیل original article <span style="font-size:12pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">سابقه و هدف:</span></span></span></b><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black"> کاندیدیازیس یک بیماری قارچی شایع در دهان است. اخیراً، به دلیل مقاومت دارویی و عوارض شناخته شده داروها، تمایل به استفاده از گیاهان دارویی رو به افزایش است. این مطالعه با هدف تعیین اثر ضد قارچی عصاره‌های آبی و الکلی پالپ و پوست موز (گروه </span></span></span><span dir="LTR" style="font-size:11.0pt"><span style="color:black">Musa AAA</span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">، زیرگروه </span></span></span><span dir="LTR" style="font-size:11.0pt"><span style="color:black">Cavendish</span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">) برکاندیدا آلبیکنس استاندارد انجام گرفت.</span></span></span><span dir="LTR" style="font-size:11.0pt"><span style="color:black"></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">مواد و روش ها:</span></span></span></b><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black"> در این مطالعه تجربی- آزمایشگاهی ، عصاره‌های الکلی و آبی پالپ و پوست موز (</span></span></span><span dir="LTR" style="font-size:11.0pt"><span style="color:black">Cavendish banana</span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">) با غلظتهای </span></span></span><span dir="LTR" style="font-size:11.0pt"><span style="color:black">mg/ml 5</span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">، </span></span></span><span dir="LTR" style="font-size:11.0pt"><span style="color:black">2/5</span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">، </span></span></span><span dir="LTR" style="font-size:11.0pt"><span style="color:black">1/25</span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">، </span></span></span><span dir="LTR" style="font-size:11.0pt"><span style="color:black">0/62</span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black"> و 0/31 تهیه شد.برای ارزیابی اثرات ضد قارچی عصاره‌ها از روش انتشار در آگار (</span></span></span><span dir="LTR" style="font-size:11.0pt"><span style="color:black">Disc Diffusion</span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">) استفاده شد. میزان </span></span></span><span dir="LTR" style="font-size:11.0pt"><span style="color:black">MIC</span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black"> و </span></span></span><span dir="LTR" style="font-size:11.0pt"><span style="color:black">MFC</span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black"> عصاره ها در مقایسه با نیستاتین تعیین شدند.برای تجزیه و تحلیل داده ها از نرم افزار&nbsp;&nbsp; </span></span></span><span dir="LTR" style="font-size:11.0pt"><span style="color:black">SPSS</span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">&nbsp; ورژن 26 و آزمون </span></span></span><span dir="LTR" style="font-size:11.0pt"><span style="color:black">repeated measures ANOVA</span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black"> استفاده شد.</span></span></span><span dir="LTR" style="font-size:11.0pt"><span style="color:black"></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">یافته ها:</span></span></span></b><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black"> بالاترین میانگین قطر هاله عدم رشد در همه گروه ها به ترتیب متعلق به نیستاتین، غلظت های </span></span></span><span dir="LTR" style="font-size:11.0pt"><span style="color:black">mg/ml </span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">5، </span></span></span><span dir="LTR" style="font-size:11.0pt"><span style="color:black">mg/ml </span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">2/5، </span></span></span><span dir="LTR" style="font-size:11.0pt"><span style="color:black">mg/ml </span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">1/25، </span></span></span><span dir="LTR" style="font-size:11.0pt"><span style="color:black">mg/ml </span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">0/62 و </span></span></span><span dir="LTR" style="font-size:11.0pt"><span style="color:black">mg/ml </span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">0/31 بود. میانگین قطر هاله عدم رشد عصاره های پوست موز و عصاره های پالپ موز تفاوت معناداری با یکدیگر نداشت (09/0=</span></span></span><span dir="LTR" style="font-size:11.0pt"><span style="color:black">p</span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">). اما میانگین قطر هاله عدم رشد عصاره های الکلی بطور معناداری بالاتر از عصاره های آبی بود (0/001></span></span></span><span dir="LTR" style="font-size:11.0pt"><span style="color:black">p</span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">). میانگین قطر هاله‌ی عدم رشد در تمامی غلظت‌ها بطور معناداری پایین تر از نیستاتین بود (0/05></span></span></span><span dir="LTR" style="font-size:11.0pt"><span style="color:black">p</span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">).</span></span></span><span dir="LTR" style="font-size:11.0pt"><span style="color:black"></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">نتیجه گیری:</span></span></span></b><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black"> عصاره آبی و الکلی موز (گروه </span></span></span><span dir="LTR" style="font-size:11.0pt"><span style="color:black">Musa AAA</span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">، زیرگروه </span></span></span><span dir="LTR" style="font-size:11.0pt"><span style="color:black">Cavendish</span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">) قادر به مهار رشد کاندیدا آلبیکنس بودند، هرچند میزان تاثیر آنها کمتر از نیستاتین بود. </span></span></span></span></span></span></span><br> &nbsp; <span style="font-size:12pt"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt">Background and Aim:</span></b> <span style="font-size:10.0pt">Candidiasis is a common fungal disease in the oral cavity. Recently, due to drug resistance and known side effects of medications, there has been an increasing tendency toward the use of medicinal plants. This study aimed to evaluate antifungal effect of aqueous and alcoholic extracts of banana pulp and banana peel (Musa AAA group, Cavendish subgroup) on standard Candida Albicans. </span></span></span><br> <span style="font-size:12pt"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt">Materials and Methods:</span></b> <span style="font-size:10.0pt">In this in vitro study, aqueous and alcoholic extracts of banana pulp and peel (Cavendish banana) were prepared at concentrations of 5, 2.5, 1.25, 0.62, and 0.31 mg/ml (n=32). The antifungal effects of the extracts were assessed using the disc diffusion method on agar. The minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of the extracts were determined in comparison with nystatin. For analyzing data SPSS software version 26 and repeated measures ANOVA test was used.</span></span></span><br> <span style="font-size:12pt"><span new="" roman="" style="font-family:" times=""><b><i><span style="font-size:9.0pt">Results</span></i></b><span style="font-size:9.0pt">: </span><span style="font-size:10.0pt">The highest mean diameter of the inhibition zone in all groups belonged to nystatin, concentrations of 5 mg/ml, 2.5 mg/ml, 1.25 mg/ml, 0.62 mg/ml, and 0.31 mg/ml, respectively. There was no significant difference in the mean inhibition zone diameters between banana peel extracts and banana pulp extracts (p=0.09). However, the mean inhibition zone diameters of alcoholic extracts were significantly higher than those of aqueous extracts (p<0.001). The mean inhibition zone diameters of all concentrations were significantly lower than that of nystatin (p<0.05).</span></span></span><br> <span style="font-size:12pt"><span new="" roman="" style="font-family:" times=""><b><i><span style="font-size:9.0pt">Conclusion</span></i></b><span style="font-size:9.0pt">: </span><span style="font-size:10.0pt">Aqueous and alcoholic extracts of banana (Musa AAA group, Cavendish subgroup) inhibited the growth of Candida Albicans, although their efficacy was lower than that of nystatin.</span></span></span><br> &nbsp; کاندیدا آلبیکنس, پوست موز, پالپ موز,Cavendish Musa Candida Albicans, Banana Peel, Banana Pulp, Cavendish Musa 200 210 http://jrds.ir/browse.php?a_code=A-10-1847-1&slc_lang=fa&sid=1 Shima Ariaei شیما آریایی Shideeeeeee@gmail.com 100319475328460021646 100319475328460021646 No دندانپزشک عمومی Mehrnoosh Rezvan Behbahani Behbahani مهرنوش رضوان بهبهانی Mehrnooshrez1@gmail.com 100319475328460021647 100319475328460021647 No دندانپزشک عمومی Nayere Amini نیره امینی Amini29@yahoo.com 100319475328460021648 100319475328460021648 Yes عضو هیئت علمی گروه سلامت دهان و دندانپزشکی اجتماعی، دانشکده دندانپزشکی، دانشگاه علوم پزشکی آزاد اسلامی تهران، تهران، ایران.