fa مقایسه اثر عصاره اتانولی سیاه دانه و نیستاتین بر روی کاندیدا آلبیکنس در شرایط آزمایشگاهی بیماری دهان Oral Medicine مطالعه اصیل original article <p dir="rtl"><span style="font-size:11pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:115%"><span sans-serif="" style="font-family:Calibri,"><span style="color:black"><b><span b="" lang="FA" nazanin="" style="font-family:">سابقه و هدف:</span></b> <span b="" lang="AR-SA" nazanin="" style="font-family:">عفونت های دهانی ناشی از گونه</span><span b="" lang="AR-SA" nazanin="" style="font-family:">‌</span><span b="" lang="AR-SA" nazanin="" style="font-family:">های کاندیدا به طور وسیع در سراسر جهان در حال افزایش است</span><span b="" lang="AR-SA" nazanin="" style="font-family:">. </span><span b="" lang="AR-SA" nazanin="" style="font-family:">یکی از درمان های موضعی رایج مربوط به کاندیدیازیس دهانی، داروی نیستاتین می باشد که دارای فعالیت ضد قارچی و ضد میکروبی است</span><span b="" lang="AR-SA" nazanin="" style="font-family:">. </span><span b="" lang="AR-SA" nazanin="" style="font-family:">درمان های ضد قارچی معمول دارای اثرات جانبی متعددی بوده و مقاومت دارویی و عود مکرر عفونت کاندیدیایی را موجب می</span><span b="" lang="AR-SA" nazanin="" style="font-family:">‌</span><span b="" lang="AR-SA" nazanin="" style="font-family:">شوند</span><span b="" lang="AR-SA" nazanin="" style="font-family:">. </span><span b="" lang="AR-SA" nazanin="" style="font-family:">استفاده از عصاره های گیاهی می تواند جایگزین مناسبی برای داروهای سنتتیک مانند نیستاتین باشند</span><span b="" lang="AR-SA" nazanin="" style="font-family:">. </span><span b="" lang="AR-SA" nazanin="" style="font-family:">هدف این مطالعه، مقایسه آزمایشگاهی اثر ضدقارچی عصاره سیاه دانه با نیستاتین بر کاندیدا آلبیکنس بود</span><span b="" lang="AR-SA" nazanin="" style="font-family:">.</span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:115%"><span sans-serif="" style="font-family:Calibri,"><span style="color:black"><b><span b="" lang="AR-SA" nazanin="" style="font-family:">مواد و روش ها:</span></b> <span b="" lang="AR-SA" nazanin="" style="font-family:">این بررسی به روش تجربی-آزمایشگاهی انجام شد، سویه استاندارد قارچ (</span>PTCC5027<span b="" lang="AR-SA" nazanin="" style="font-family:">) از سازمان پژوهش های علمی و صنعتی ایران تهیه گردید. عصاره اتانولی سیاه</span> <span b="" lang="AR-SA" nazanin="" style="font-family:">دانه به روش خیساندن بدست آمد. به منظور ارزیابی خواص ضد قارچی از روش دیسک گذاری در محیط کشت مولر هینتون آگار استفاده شد. عصاره با رقت های</span> <span b="" lang="AR-SA" nazanin="" style="font-family:">09/0 ، 19/0، 39/0، 78/0، 56/1، 12/3، 25/6، 5/12، 50/25 &nbsp;بر روی کاندیدا اثر داده شد و حداقل غلظت مهارکنندگی (MIC</span>( <span dir="LTR" lang="AR-SA" new="" roman="" style="font-family:" times="">Minimum</span> <span dir="LTR" lang="AR-SA" new="" roman="" style="font-family:" times="">Inhibitory</span> <span dir="LTR" lang="AR-SA" new="" roman="" style="font-family:" times="">Concentration</span><span b="" lang="AR-SA" nazanin="" style="font-family:"> و حداقل غلظت کشندگی</span> <span b="" lang="AR-SA" nazanin="" style="font-family:">قارچی (MFC)</span> <span dir="LTR" lang="AR-SA" new="" roman="" style="font-family:" times="">Minimum Fungicidal Concentration</span><span b="" lang="AR-SA" nazanin="" style="font-family:"> با استفاده از تکنیک </span><span dir="LTR" lang="AR-SA" new="" roman="" style="font-family:" times="">Microdilution broth</span><span b="" lang="AR-SA" nazanin="" style="font-family:"> و </span><span dir="LTR" lang="AR-SA" new="" roman="" style="font-family:" times="">Macrodilution broth</span><span b="" lang="AR-SA" nazanin="" style="font-family:"> محاسبه گردید. هر آزمایش ۳ بار تکرار گردید و داده های بدست آمده با نرم افزار </span>SPSS <span b="" lang="AR-SA" nazanin="" style="font-family:">&nbsp;نسخه ۲۵ و </span>T-test <span b="" lang="AR-SA" nazanin="" style="font-family:">&nbsp;مورد تجزیه و تحلیل قرار گرفت. </span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span arial="" ms="" style="font-family:" unicode=""><span style="color:black"><b><span b="" lang="FA" nazanin="" style="font-family:">یافته&shy;ها:</span></b> <span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">میانگین قطر هاله عدم رشد ناشی از عصاره اتانولی سیاه دانه بر روی قارچ، 5/19 میلی متر گزارش شد</span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">. </span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">میانگین قطر هاله عدم رشد نیستاتین بر روی قارچ، 5/24 میلی متر گزارش شد</span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">. </span></span><span lang="AR-SA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:">MIC </span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">و </span></span><span lang="AR-SA" style="font-size:11.0pt"><span new="" roman="" style="font-family:" times="">MFC</span></span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">عصاره اتانولی در هر دو روش </span></span><span dir="LTR" lang="AR-SA" style="font-size:11.0pt"><span new="" roman="" style="font-family:" times="">Microdilution broth</span></span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">و </span></span><span dir="LTR" lang="AR-SA" style="font-size:11.0pt"><span new="" roman="" style="font-family:" times="">Macrodilution broth</span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> بر روی قارچ56/1و 12/3 صدم میلی گرم</span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">/</span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">میلی لیتر بدست آمد</span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">.</span></span><span lang="AR-SA" style="font-size:11.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span arial="" ms="" style="font-family:" unicode=""><span style="color:black"><b><span b="" lang="FA" nazanin="" style="font-family:">نتیجه گیری</span></b><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">: </span></span>&nbsp;&nbsp;<span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">با توجه به نتایج حاصل از تحقیق حاضر، عصاره اتانولی گیاه سیاه دانه اثر مهاری مثبتی بر روی قارچ کاندیدا آلبیکنس دارد ولی اثر ضدقارچی نیستاتین نسبت به عصاره اتانولی سیاه دانه به طور معناداری بیشتر می باشد</span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">.</span></span><span lang="AR-SA" style="font-size:11.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span></p> <div style="border-bottom:solid windowtext 1.0pt; padding:0in 0in 1.0pt 0in"></div> <p><span style="font-size:12pt"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:11.0pt"><span style="color:black">Abstract</span></span></b></span></span><br> <span style="font-size:12pt"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt">Background and&nbsp; Aims:</span></b> <span style="font-size:10.0pt">Oral infections caused by Candida species are increasing worldwide. One of the common topical treatments related to oral candidiasis is nystatin, which has anti-fungal and antimicrobial activity. Common anti-fungal treatments have many side effects and cause drug resistance and repeated recurrence of Candida infection. The use of plant extracts can be a suitable alternative to synthetic drugs such as nystatin. The aim of this study was to compare the anti-fungal effect of Nigella Sativa with nystatin on Candida albicans.</span></span></span><br> <span style="font-size:12pt"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:11.0pt">Materials and Methods: </span></b><span style="font-size:9.0pt">An experimental study was conducted by referring to the Scientific and Industrial Research Organization of Iran and the standard strain of Candida albicans with PTCC5027 was provided. Ethanol soaking method was used to obtain the Nigella sativa ethanolic extract. Fungal suspension with 0.5 McFarland concentration was cultured in a broth medium. To investigate the anti-candida properties of the Nigella sativa Linn ethanolic extract , Disk diffusion technique was done in a Potato dextrose agar by uniformly inoculating the fungal suspension on the culture medium of potato dextrose agar then using a 100 &mu;l micropipette, the Nigella sativa Linn extract with a concentration of 100 mg/ml and Nystatin in a dose of 0.06 mg was poured on a sterile blank disk and put in the plates and , subsequently the growth inhibition zone of the extract was measured. By diluting the extract and nystatin to &nbsp;0.09, 0.19, 0.39, 0.78, 1.56, 3.12, 6.25, 12.5, 25.50 the Minimum Inhibitory Concentration (MIC) and the Minimum fungicidal concentration (MFC) were obtained using both Microdilution broth and Macrodilution broth. Each experiment was repeated 3 times and the obtained data were statistically analyzed using SPSS ver:25 software and t-test exam. </span></span></span><br> <span style="font-size:12pt"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt">Results:</span></b><b> </b><span style="font-size:10.0pt">The length of the inhibition zone measured from the Nigella sativa Linn extract was 19.5mm respectively and the length of the inhibition zone obtained by Nystatin was 24.5 mm respectively. Studying the results of the dilution test in both techniques concluded from the Nigella sativa Linn extract showed that the Nigella sativa Linn ethanolic extract at a concentration of 1.56 mg /ml has the least Minimum Inhibitory Concentration (MIC) and at a concentration of 3.12 mg/ml it has the lowest Minimum Fungicidal Concentration (MFC). Statistical analysis (T-test) showed that the difference in length of the Nigella sativa Linn inhibition zone with Nystatin (P <0.05) was significant.</span></span></span><br> <span style="font-size:12pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><b><span style="font-size:11.0pt">Conclusion:</span></b> <span style="font-size:10.0pt">According to our findings, it seems that the Nigella sativa Linn ethanolic extract is quite effective on the growth of Candida albicans and has an antifungal effect. However, the antifungal effect of Nystatin is significantly higher than the Nigella sativa Linn ethanolic extract. </span></span></span></span><br> <span style="font-size:12pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-family:"Arial","sans-serif""></span></span></span></span><br> &nbsp;</p> قارچ کاندیدا آلبیکنس, عصاره سیاه دانه, نیستاتین Candida albicans, Nigella sativa Linn, Nystatin 133 141 http://jrds.ir/browse.php?a_code=A-10-1855-1&slc_lang=fa&sid=1 Armin Dizajian Fard Zanjani آرمین دیزجیان!فرد!زنجانی 100319475328460020703 100319475328460020703 No Azad university دندانپزشک عمومی Hanieh Jadidi هانیه جدیدی hanieh.jadidi@yahoo.com 100319475328460020704 100319475328460020704 No Azad university تخصصی کودکان،‌ دانشکده دندانپزشکی، دانشگاه علوم پزشکی آزاد اسلامی تهران Peyvand Moeini پیوند معینی 100319475328460020705 100319475328460020705 No Azad university - متخصص دندانپزشکی کودکان Sepideh Majzoubi سپیده مجذوبی 100319475328460020706 100319475328460020706 Yes گروه کودکان، دانشکده دندانپزشکی، دانشگاه علوم پزشکی آزاد اسلامی تهران