journal of research in dental sciences
مجله تحقیق در علوم دندانپزشکی
J Res Dent Sci
Medical Sciences
http://jrds.ir
1
admin
2008-4676
2228-7353
8
10.66224/jrds
14
13
fa
jalali
1403
3
1
gregorian
2024
6
1
21
2
online
1
fulltext
fa
بررسی تأثیر اشعه X در تصویربرداری CBCT بر میزان تغییرات هسته ای و Repair Index سلولهای مخاط باکال
The effect of X-rays in CBCT on the amount of nuclear changes and Repair Index in the buccal mucosa
رادیولوژی
Radiology
مطالعه اصیل
original article
<span style="font-size:12pt"><span style="tab-stops:right 1.75in"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><span lang="AR-SA" style="font-family:"B Nazanin""><span style="color:black">خلاصه:</span></span></b><b><span lang="FA" style="font-family:"B Nazanin""><span style="color:black"></span></span></b></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="line-height:115%"><span style="text-autospace:none"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><span lang="AR-SA" style="font-family:"B Nazanin""><span style="color:black">سابقه و هدف</span></span></b><span lang="AR-SA" style="font-family:"B Nazanin""><span style="color:black">: </span></span><span lang="FA" style="font-size:11.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""><span style="color:black">هدف این مطالعه تعیین تأثیر اکسپوژر با اشعه </span></span></span></span><span dir="LTR" style="font-size:11.0pt"><span style="line-height:115%"><span style="color:black">X</span></span></span><span lang="FA" style="font-size:11.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""><span style="color:black"> در تصویربرداری </span></span></span></span><span dir="LTR" style="font-size:11.0pt"><span style="line-height:115%"><span style="color:black">CBCT</span></span></span><span lang="FA" style="font-size:11.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""><span style="color:black">بر میزان تغییرات هسته ای و </span></span></span></span><span dir="LTR" style="font-size:11.0pt"><span style="line-height:115%"><span style="color:black">Repair index</span></span></span><span lang="FA" style="font-size:11.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""><span style="color:black"> سلول­های مخاط باکال در مراجعین به بخش رادیولوژی دهان و فک و صورت دانشگاه آزاد تهران در سال 1401 بود. </span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="line-height:115%"><span style="text-autospace:none"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><span lang="FA" style="font-family:"B Nazanin""><span style="color:black">مواد و روش ها:</span></span></b> <span lang="FA" style="font-size:11.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""><span style="color:black">مطالعه حاضر به روش تجربی از نوع مقایسه قبل و بعد بود که بر روی 63 نفر از بیماران واجد شرایط انجام شد. سلول</span></span></span></span><span lang="FA" style="font-size:11.0pt"><span style="line-height:115%"><span style="color:black">¬</span></span></span><span lang="FA" style="font-size:11.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""><span style="color:black">های مخاط باکال، قبل و 10 روز بعد از اکسپوژر با اشعه </span></span></span></span><span dir="LTR" style="font-size:11.0pt"><span style="line-height:115%"><span style="color:black">X</span></span></span><span style="font-size:11.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""><span style="color:black"> نمونه برداری شدند. سپس از رنگ آمیزی </span></span></span></span><span dir="LTR" style="font-size:11.0pt"><span style="line-height:115%"><span style="color:black">papanicolaou</span></span></span><span lang="FA" style="font-size:11.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""><span style="color:black"> برای سنجش میکرونوکلئوس و سایر تغییرات هسته ای استفاده شده و مشاهده نمونه ها با میکروسکوپ نوری با بزرگنمایی400× انجام گرفت. تصاویر </span></span></span></span><span dir="LTR" style="font-size:11.0pt"><span style="line-height:115%"><span style="color:black">CBCT</span></span></span><span lang="FA" style="font-size:11.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""><span style="color:black"> توسط دستگاه </span></span></span></span><span dir="LTR" style="font-size:11.0pt"><span style="line-height:115%"><span style="color:black">Papaya 3D Plus</span></span></span><span lang="FA" style="font-size:11.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""><span style="color:black"> با دو </span></span></span></span><span dir="LTR" style="font-size:11.0pt"><span style="line-height:115%"><span style="color:black">FOV</span></span></span><span lang="FA" style="font-size:11.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""><span style="color:black"> کوچک و بزرگ تهیه شدند. جهت مقایسه هر کدام از شاخص­ها قبل و بعد از تابش از آزمون </span></span></span></span><span dir="LTR" style="font-size:11.0pt"><span style="line-height:115%"><span style="color:black">repeated measure ANOVA</span></span></span><span lang="FA" style="font-size:11.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""><span style="color:black"> با در نظرگرفتن جنس و </span></span></span></span><span dir="LTR" style="font-size:11.0pt"><span style="line-height:115%"><span style="color:black">FOV</span></span></span><span lang="FA" style="font-size:11.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""><span style="color:black"> به عنوان </span></span></span></span><span dir="LTR" style="font-size:11.0pt"><span style="line-height:115%"><span style="color:black">between subject factor</span></span></span><span lang="FA" style="font-size:11.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""><span style="color:black"> در صورت تبعیت داده­ها از توزیع نرمال استفاده شد.</span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="line-height:115%"><span style="text-autospace:none"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><span lang="AR-SA" style="font-family:"B Nazanin""><span style="color:black">یافته ها</span></span></b><span lang="AR-SA" style="font-family:"B Nazanin""><span style="color:black">: در میزان </span></span><span dir="LTR" style="font-size:11.0pt"><span style="line-height:115%"><span style="color:black">Micronucleus</span></span></span> <span lang="FA" style="font-family:"B Nazanin""><span style="color:black">، </span></span><span dir="LTR" style="font-size:11.0pt"><span style="line-height:115%"><span style="color:black">Karyorrhex</span></span></span><span dir="LTR" style="font-family:"Calibri","sans-serif""><span style="color:black">i</span></span><span lang="FA" style="font-family:"B Nazanin""><span style="color:black"> ، </span></span><span dir="LTR" style="font-size:11.0pt"><span style="line-height:115%"><span style="color:black">Broken egg</span></span></span><span lang="AR-SA" style="font-size:11.0pt"><span style="line-height:115%"><span style="color:black">و </span></span></span><span dir="LTR" style="font-size:11.0pt"><span style="line-height:115%"><span style="color:black">Karyolysis</span></span></span><span style="font-family:"B Nazanin""><span style="color:black"> قبل و بعد از اکسپوژر اشعه در </span></span><span dir="LTR" style="font-size:11.0pt"><span style="line-height:115%"><span style="color:black">CBCT</span></span></span><span lang="FA" style="font-family:"B Nazanin""><span style="color:black"> در سلول های مخاط باکال افزایش معنی داری دیده شد. به ترتیب (001/0></span></span><span dir="LTR" style="font-family:"Calibri","sans-serif""><span style="color:black">P</span></span><span lang="FA" style="font-family:"B Nazanin""><span style="color:black">) و (01/0=</span></span><span dir="LTR" style="font-family:"Calibri","sans-serif""><span style="color:black">P</span></span><span lang="FA" style="font-family:"B Nazanin""><span style="color:black">) اما تفاوت معنی داری در </span></span><span dir="LTR" style="font-size:11.0pt"><span style="line-height:115%"><span style="color:black">Repair Index</span></span></span> <span lang="FA" style="font-family:"B Nazanin""><span style="color:black"> مشاهده نشد. (054/0=</span></span><span dir="LTR" style="font-family:"Calibri","sans-serif""><span style="color:black">P</span></span><span lang="FA" style="font-family:"B Nazanin""><span style="color:black">) </span></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="line-height:115%"><span style="text-autospace:none"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><span lang="AR-SA" style="font-family:"B Nazanin""><span style="color:black">ﻧﺘﻴﺠﻪ ﮔﻴﺮی</span></span></b><span lang="AR-SA" style="font-family:"B Nazanin""><span style="color:black">:</span></span><span lang="AR-SA" style="font-family:"B Nazanin""><span style="color:black"> تصویر</span></span><span lang="AR-SA" style="font-size:11.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""><span style="color:black">برداری </span></span></span></span><span dir="LTR" style="font-size:11.0pt"><span style="line-height:115%"><span style="font-family:"Times New Roman Bold","serif""><span style="color:black">CBCT</span></span></span></span><span lang="AR-SA" style="font-size:11.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""><span style="color:black"> میتواند اثرات سیتوتوکسیک و ژنوتوکسیک را بر سلولهای مخاط باکال داشته باشد. بنابراین، </span></span></span></span><span dir="LTR" style="font-size:11.0pt"><span style="line-height:115%"><span style="font-family:"Times New Roman Bold","serif""><span style="color:black">CBCT</span></span></span></span><span lang="AR-SA" style="font-size:11.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""><span style="color:black"> فقط باید در صورت لزوم تجویز شود.</span></span></span></span><span lang="FA" style="font-size:11.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""><span style="color:black"></span></span></span></span></span></span></span></span></span></span><br>
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<span style="font-size:12pt"><span style="font-family:"Times New Roman","serif""><span style="font-size:10.0pt">Abstract</span></span></span><br>
<span style="font-size:12pt"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:10.0pt">Background and Aim:</span></b><span style="font-size:10.0pt"> The purpose of this study is to determine the effect of X-ray exposure in CBCT imaging on the amount of nuclear changes and repair index of buccal mucosa cells in the patients of oral and maxillofacial radiology department of Tehran Azad University in 2022. <b><i></i></b></span></span></span><br>
<span style="font-size:12pt"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:10.0pt">Materials and methods:</span></b><span style="font-size:10.0pt"> he present study is an experimental research, comparing before and after, which was conducted on 63 eligible patients. Buccal mucosa cells were examine. once before and once 10 days after X-ray exposure, Then Papanicolaou staining was used to measure the micronucleus and other nuclear changes and observation was done with a light microscope with 400× magnification. CBCT images by Papaya 3D Plus device (Genoray, Korea) with two FOVs, small (4 x 4 cm) and large (14 x 14 cm) and Voxel Size: 0.175 mm with exposure conditions of 60-90 kV and 4-12 mA were prepared.<span style="background:yellow"> <b><i></i></b></span></span></span></span><br>
<span style="font-size:12pt"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:10.0pt">Results:</span></b><span style="font-size:10.0pt"> Statistically significant increase in Micronucleus (p<0.001), Karyorrhexis (p<0.001), Karyolysis (p=0.01) and Broken egg (p<0.001) before and after X-ray exposure. But there is no statistically significant difference in the amount of Repair index (p=0.054) </span></span></span><br>
<span style="font-size:12pt"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:10.0pt">Conclusion: </span></b><span style="font-size:10.0pt">The present study shows that CBCT imaging can induce cytotoxic and genotoxic effects on buccal mucosa cells. Therefore, CBCT should only be prescribed when necessary because it cannot be considered a risk-free procedure<b><i>.</i></b></span></span></span><br>
<span style="font-size:12pt"><span style="font-family:"Times New Roman","serif""><span style="font-size:10.0pt"><span style="background:yellow"></span></span></span></span><br>
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توموگرافی کامپیوتری با پرتو مخروطی , سلولهای مخاط باکال, تست میکرونوکلئوس, تست پاپانیکولائو, تغییرات هستهای, Repair Index
CBCT, buccal mucosa cells, nuclear changes, Repair Index
107
115
http://jrds.ir/browse.php?a_code=A-10-1803-1&slc_lang=fa&sid=1
parissa
shahmohammadi
پریسا
شاه محمدی
parissashahmohammadi91@gmail.com
100319475328460020631
0009-0005-6313-3438
No
رادیولوژی فک و صورت، دانشکده دندانپزشکی، دانشگاه علوم پزشکی آزاد اسلامی تهران
ahmadreza
talaeipor
احمدرضا
طلائی پور
100319475328460020632
100319475328460020632
Yes
گروه رادیولوژی دهان، فک و صورت، دانشکده دندانپزشکی، دانشگاه علوم پزشکی آزاد اسلامی تهران
maryam
jolehar
مریم
جولهر
100319475328460020633
100319475328460020633
No
گروه آسیب شناسی دهان و فک وصورت، دانشکده دندانپزشکی، دانشگاه علوم پزشکی آزاد اسلامی تهران
shirin
sakhdari
شیرین
سخدری
100319475328460020634
100319475328460020634
No
گروه رادیولوژی دهان، فک و صورت، دانشکده دندانپزشکی، دانشگاه علوم پزشکی آزاد اسلامی تهران