fa تاثیر ﺳﻪ ﻧوع براکت ارتودنسی فلزی، سرامیکی و self ligating بر میزان چسبندگی استرپتوکوک موتانس اورتو Ortho مطالعه اصیل original article <p><strong><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">سابقه و هدف:</span></span></strong><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> ﺑﺎ ﺗﻮﺟﻪ ﺑﻪ ﮔﺴﺘﺮش درﻣﺎن ﻫﺎی ارﺗﻮدﻧﺴﻰ و ﺗﻐﻴﻴﺮ ﻓﻠﻮر دﻫﺎن ﻣﺘﻌﺎﻗﺐ درﻣﺎن ارﺗﻮدﻧﺴﻰ و در ﻧﺘﻴﺠﻪ اﻓﺰاﻳﺶ ﺑﺮوز ﺿﺎﻳﻌﺎت ﺳﻔﻴﺪ ﻣﻴﻨﺎﻳﻰ در ﺑﻴﻤﺎران ﺗﺤﺖ درﻣﺎن ارﺗﻮدﻧﺴﻰ و وﺟﻮد آﻣﺎر ﻣﺘﻨﺎﻗﺾ ﭘﻴﺮاﻣﻮن ﺗﺎﺛﻴﺮ نوع ﺑﺮاﻛﺖ ﻫﺎ ﺑﺮ ﻣﻴﺰان ﭼﺴﺒﻨﺪﮔﻰ ﻣﻴﻜﺮو ارﮔﺎﻧﻴﺰم ﻫﺎ، اﻳﻦ ﺗﺤﻘﻴﻖ ﺑﺎ ﻫﺪف ﻣﻘﺎﻳﺴﻪ ﺗﺎﺛﻴﺮ ﺳﻪ ﻧﻮع ﺑﺮاﻛﺖ ارﺗﻮدﻧﺴﻰ (ﻓﻠﺰی، ﺳﺮاﻣﻴﻜﻰ و </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">self-ligating</span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">) ﺑﺮ ﻣﻴﺰان رﺷﺪ و ﭼﺴﺒﻨﺪﮔﻰ اﺳﺘﺮﭘﺘﻮﻛﻮک ﻣﻮﺗﺎﻧﺲ اﻧﺠﺎم ﺷﺪ.</span></span><br> <strong><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">مواد و روش ها: </span></span></strong><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">ﺗﺤﻘﻴﻖ ﺑﻪ روش تجربی و ﺗﺤﺖ ﺷﺮاﻳﻂ </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">in vitro</span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">&nbsp; ﺑﺮ روی ٦٠ ﻧﻤﻮﻧﻪ اﻧﺠﺎم ﺷﺪ. ﺳﻪ ﻧﻮع ﺑﺮاﻛﺖ ﻓﻠﺰی، ﺳﺮاﻣﻴﻜﻰ و </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">self-ligating</span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> ﻣﺮﺑﻮط ﺑﻪ دﻧﺪان ﭘﺮه ﻣﻮﻟﺮ ﺑﻪ ﻣﻨﻈﻮر ﻣﻘﺎﻳﺴﻪ ﭼﺴﺒﻨﺪﮔﻰ اﺳﺘﺮﭘﺘﻮﻛﻮک ﻣﻮﺗﺎﻧﺲ ﻣﻮرد ﻣﻄﺎﻟﻌﻪ ﻗﺮار ﮔﺮﻓﺖ.&nbsp; ﻫﺮ ﻛﺪام از ﺑﺮاﻛﺖ ﻫﺎ درون ﭘﻠﻴﺖ ﻫﺎی ﺣﺎوی ١٥ ﺳﻰ ﺳﻰ از ﻣﺤﻴﻂ ﻛﺸﺖ ﻧﻮﺗﺮﻳﻨﺖ ﺑﺮاث اﺳﺘﺮﻳﻞ شده ﻫﻤﺮاه ﺑﺎ ٢٠% ﺳﻮﻛﺮوز ﻗﺮار داده ﺷﺪند.&nbsp; ﺳﭙﺲ ﺑﻪ ﻫﺮ ﻛﺪام از ﭘﻠﻴﺖ ﻫﺎ ٥ ﺳﻰ ﺳﻰ از ﺳﻮﺳﭙﺎﻧﺴﻴﻮن ﻣﻴﻜﺮوﺑﻰ ﺗﻬﻴﻪ ﺷﺪه اﺿﺎﻓﻪ ﺷﺪ. ﭘﻠﻴﺖ ﻫﺎ ﺑﻪ ﻣﺪت ٥ روز در اﻧﻜﻮﺑﺎﺗﻮر ٣٧ درﺟﻪ ﺳﺎﻧﺘﻰ ﮔﺮاد اﻧﻜﻮﺑﻪ ﺷﺪﻧﺪ. ﺳﭙﺲ ﺑﻌﺪ از ﺧﺎرج ﺳﺎزی ﻣﺤﻴﻂ ﻣﺎﻳﻊ ﻧﻮﺗﺮﻳﻨﺖ ﺑﺮاث از ﭘﻠﻴﺖ ﻫﺎ، ﭘﻠﻴﺖ ﻫﺎ ﺳﺮو ﺗﻪ ﺷﺪه و ﺗﻌﺪاد ﻛﻠﻮﻧﻰ ﻫﺎی ﭼﺴﺒﻴﺪه ﺑﻪ ﭘﻠﻴﺖ ﺑﻪ ﺻﻮرت ﭼﺸﻤﻰ ﺷﻤﺎرش ﺷﺪه و ﺑﺮ اﺳﺎس </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">colony forming unit</span></span></span> <span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">(CFU)</span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> ﺑﻴﺎن ﺷﺪﻧﺪ. در مرحله بعدی آزمایش به منظور تایید مرحله اول مطالعه براکت ها درون محیط بلود آگار قرار گرفته و کلونی های حاصل مورد شمارش قرار گرفته که نتایج حاصله از آن تایید کننده مرحله اول بود. داده ﻫﺎ ﺗﻮﺳﻂ آزﻣﻮن آﻣﺎری </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">ANOVA</span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> ﻣﻮرد ﻗﻀﺎوت ﻗﺮار گرفتند.</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;"></span></span></span><br> <strong><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">یافته ها: </span></span></strong><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">ﺗﺤﻘﻴﻖ روی ٦٠ ﻧﻤﻮﻧﻪ ﺑﺮاﻛﺖ از ﺳﻪ نوع ﻓﻠﺰی، ﺳﺮاﻣﻴﻜﻰ و </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">self-ligating</span></span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">اﻧﺠﺎم ﺷﺪ. نتایج مطالعه نشان داد که ﺑﻴﺸﺘﺮﻳﻦ ﻣﻴﺰان ﭼﺴﺒﻨﺪﮔﻰ اﺳﺘﺮﭘﺘﻮﻛﻮک ﻣﻮﺗﺎﻧﺲ به ترتیب ﻣﺘﻌﻠﻖ ﺑﻪ ﺑﺮاﻛﺖ </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">self-ligating</span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> ﺑﻪ ﻣﻴﺰان ٠١</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">/</span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">١٥</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">&plusmn;</span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> ٢٥</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">/</span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">٥١ و ﺑﻌﺪ ﺑﺮاﻛﺖ ﻓﻠﺰی ﺑﻪ ﻣﻴﺰان ١</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">/</span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">٧</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">&plusmn;</span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> ٨</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">/</span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">٢١ و ﺑﺮاﻛﺖ ﺳﺮاﻣﻴﻜﻰ ﺑﻪ ﻣﻴﺰان ٦</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">/</span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">٣</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">&plusmn;</span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> ٥</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">/</span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">١٥ﺑﻮد و تفاوت بین 3 گروه معنی دار بود (٠٠١</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">/</span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">٠</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">(P<</span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">. در ضمن نتایج حاصله با آزمایش نمو.نه ها در محیط کشت بلود اگار نیز تایید شد </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">(p<0.01).</span></span></span><br> <strong><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">ﻧﺘﻴﺠﻪ ﮔﻴﺮی: </span></span></strong><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">ﻣﻴﺰان رﺷﺪ و ﭼﺴﺒﻨﺪﮔﻰ اﺳﺘﺮﭘﺘﻮﻛﻮک ﻣﻮﺗﺎﻧﺲ در ﺣﻀﻮر ﺑﺮاﻛﺖ ﻫﺎی ارﺗﻮدﻧﺴﻰ ﺑﺎ ﺟﻨﺲ ﻫﺎ و ﻃﺮاﺣﻰ ﻫﺎی ﻣﺨﺘﻠﻒ، ﻣﺘﻔﺎوت اﺳﺖ.&nbsp; بیشترین&nbsp; چسپندگی اﺳﺘﺮﭘﺘﻮﻛﻮک ﻣﻮﺗﺎﻧﺲ متعلق به ﺑﺮاﻛﺖ </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">self-ligating</span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> و کمترین میزان چسپندگی متعلق به براکت سرامیکی بود.</span></span><br> &nbsp;<br> &nbsp;<br> &nbsp;</p> <div><strong>Background and Aim: </strong>&nbsp;With increasing demand for orthodontic treatment among adults and with changing oral flora and increased number of white spot lesions around orthodontic appliances in patients undergone orthodontic treatments and also with controversial reports about the role of different brackets types on adhesion of Streptococcus mutans, this study was aimed to compare the effect of different orthodontic bracket material and design (metal, ceramic, self -ligating) on growth and adherence of streptococcus mutans.<br> <strong>Materials and methods:</strong> This experimental in vitro study was accomplished on 60 premolar bracket samples. Three types of brackets (metallic, ceramic and self-ligating) were used for comparison of streptococcus mutans adhesion. Each of the brackets was placed into small size plates containing 15cc of sterile Nutrient broth with %20 sucrose. Then 5cc of bacterial suspension were added to each plate. Plates were incubated for 5 days at 37&deg;C. Then, after removal of the nutrient broth liquid from plates, the plate was turned upside down and the number of colonies attached to the plate was counted by eye according to Colony forming unit (CFU). In order to confirm the results of the first phase of the study, the contaminated brackets were placed in the blood agar medium. The results of the second test confirmed the validity of the first step. Data were analyzed by ANOVA test.<br> <strong>Results:</strong> The results of the study showed that the maximum streptococcus mutans adhesion was belonged to self-ligating (51.25&plusmn;15.01), followed by metal (21.8&plusmn;7.1) brackets and there was lowest adhesion was to ceramic brackets (15.5&plusmn;3.6) and the differences were statistically significant (p<0.001). The second stage of the study also confirmed the results of the first stage (p<0.01).<br> <strong>Conclusion:</strong> The growth and adhesion of Streptococcus mutans is different in the presence of various orthodontics bracket material and bracket designs.&nbsp; In an in vitro setup, the self-ligating metallic brackets showed higher adhesion and growth of Streptococcus mutans than conventional twin metal brackets, followed by Ceramic brackets. &nbsp;<br> &nbsp;</div> براکت های ارتودنسی, اﺳﺘﺮﭘﺘﻮﻛﻮک ﻣﻮﺗﺎﻧﺲ, ﭼﺴﺒﻨﺪﮔﻰ پلاک orthodontic bracket,Streptococcus mutans, plaque adhesion 268 274 http://jrds.ir/browse.php?a_code=A-10-1436-2&slc_lang=fa&sid=1 MARYAM NOURBAKHSH مریم نوربخش 100319475328460018592 100319475328460018592 No دندانپزشک عمومی ALIREZA JAFARI NAEIMI علیرضا جعفری نعیمی : jafariorthodclinical@gmail.com 100319475328460018593 100319475328460018593 Yes بخش ارتودنسی، دانشکده دندانپزشکی دانشگاه علوم پزشکی آزاد اسلامی تهران MARYAM POUSTI مریم پوستی 100319475328460018594 100319475328460018594 No متخصص ارتودنسی