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Comparison of two commercial dermal matrix on biological behavior of endothelial cells (in vitro)
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Omid Maoghadas *    , EHSAN JFAFRI , Borhan Bakhshi , MOHAMAD HARANDI |
| , hmonn555@yahoo.com |
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Abstract: (5121 Views) |
Abstract
Aim and background: Behavior of endothelial cells can have significant role regarding their proliferation and attachment on acellualr dermal grafts in regeneration procedures.The purpose of this study is to evaluate the effects of two dermal scaffolds on attachment and proliferation of human umbilical vein endothelial cells.
Methods and Materials: This study was conducted on experimental approach. Human umbilical vein endothelial cells were seeded, experimentally. Two commercially available types of ADM were used. 1. Kish tissue regeneration co. 2. Knowledge Based Company of Persian Textile Products. The proliferation rate in matrix and control groups were evaluated 12,24, 84 hours and 7 days after culture using MTT 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide ) test. The morphologic evaluation was made after 24 hours incubation with Ethanol and Dehydrated Hexamethyldisilazane (HMDS) using Platinium coated drops. The surface and circularity of cells was examined by electronic microscope.
Results: The results for DAPI and MTT index was similar in 3 groups(P<0.4).After 1 week the control group rate was 2.66 ± 0.001, fist group, 2.09 ±0.001 and second group, 1.64±0.17. The result was statistically significant.(P<0.1)
Conclusion: It seems, in experimental status, both matrix was similar in endothelia cell adhesion. But, proliferation rate of endothelial cells was more in matrix 1 v.s matrix 2 after 7 days. |
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| Keywords: dermal cells, Matrix, endothelial cell, biological behavior |
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Full-Text [PDF 472 kb]
(1111 Downloads)
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Type of Study: original article |
Subject:
Perio
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