fa اثر ضد سرطانی عصاره ی آبی گل محمدی بر روی کارسینوما سلول سنگفرشی دهان اورال پاتولوژی oral patho مطالعه اصیل original article <h3 dir="RTL" style="text-align:justify"><span style="font-size:8pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span bold="" new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">سابقه و هدف:</span></span></span>&nbsp; <span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black"><span style="font-weight:normal">درمانهای متداول سرطان، معمولا عوارض پیچیده ای دارند. هم چنین مقاومت سلولهای سرطانی به درمانها نکته ویژه ای است که نیاز به درمانهای آلترناتیو منجمله استفاده از ترکیبات گیاهی را مشخص می کند. هدف این مطالعه بررسی اثر ضدسرطانی عصاره آبی گیاه گل محمدی بر روی سل لاین سرطان دهان بوده است.</span></span></span></span></span></span></span></span></span></span></h3> <h3 dir="RTL" style="text-align:justify"><span style="font-size:8pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span bold="" new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">مواد و روش ها</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">:</span></span></span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black"><span style="font-weight:normal">در این مطالعه تجربی ، اثر سمیت عصاره آبی گل محمدی با غلظتهای مختلف طی مدت زمان 48 ساعت و با تکرار آزمایش 5 بار، بر زیست پذیری سلولهای کارسینوما دهان انسان (سل لاین </span></span></span></span><span dir="LTR" style="font-size:11.0pt"><span new="" roman="" style="font-family:" times=""><span style="font-weight:normal">NCBI No#C152</span></span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black"><span style="font-weight:normal">) و سلولهای نرمال فیبروبلاست لثه (سل لاین </span></span></span></span><span dir="LTR" lang="X-NONE" style="font-size:11.0pt"><span style="color:black"><span style="font-weight:normal">HF2FF</span></span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black"><span style="font-weight:normal">)، با روش </span></span></span></span><span dir="LTR" style="font-size:11.0pt"><span new="" roman="" style="font-family:" times=""><span style="font-weight:normal">MTT</span></span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black"><span style="font-weight:normal"> بررسی و در نهایت، جذب نوری (</span></span></span></span><span dir="LTR" style="font-size:11.0pt"><span new="" roman="" style="font-family:" times=""><span style="font-weight:normal">Optical density</span></span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black"><span style="font-weight:normal">) محلول بدست آمده در طول موج </span></span></span></span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black"><span style="font-weight:normal">۵۷۰</span></span></span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black"><span style="font-weight:normal"> نانومتر با </span></span></span></span><span lang="FA" style="font-size:11.0pt"><span calibri="" style="font-family:"><span style="font-weight:normal">دستگاه</span></span></span><span dir="LTR" style="font-size:11.0pt"><span new="" roman="" style="font-family:" times=""><span style="font-weight:normal">Elisa</span></span></span> <span dir="LTR" style="font-size:11.0pt"><span new="" roman="" style="font-family:" times=""><span style="font-weight:normal">Reader</span></span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black"><span style="font-weight:normal">&nbsp; خوانده شد و نهایتا نرخ مهار رشد سلولها (درصد) بدست آمد. نتایج حاصله با استفاده از آزمون </span></span></span></span><span dir="LTR" style="font-size:11.0pt"><span new="" roman="" style="font-family:" times=""><span style="font-weight:normal">Two</span></span></span> <span dir="LTR" style="font-size:11.0pt"><span new="" roman="" style="font-family:" times=""><span style="font-weight:normal">Way</span></span></span> <span dir="LTR" style="font-size:11.0pt"><span new="" roman="" style="font-family:" times=""><span style="font-weight:normal">ANOVA</span></span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black"><span style="font-weight:normal">&nbsp; ارزیابی شد و برای مقایسه غلظتها از آزمون </span></span></span></span><span dir="LTR" style="font-size:11.0pt"><span new="" roman="" style="font-family:" times=""><span style="font-weight:normal">POST HOC Tukey</span></span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black"><span style="font-weight:normal"> استفاده شد. </span></span></span></span></span></span></span></span></span></span></h3> <h3 dir="RTL" style="text-align:justify"><span style="font-size:8pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span bold="" new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">یافته</span></span></span> <span lang="AR-SA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">ها</span></span></span><span b="" lang="AR-SA" nazanin="" style="font-family:"><span style="color:black">: </span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black"><span style="font-weight:normal">&nbsp;نتایج نشان داد که عصاره آبی گل محمدی بر روی زیست پذیری رده سلول های سرطانی نسبت به گروه کنترل منفی تاثیر داشته و باعث کاهش زیست پذیری سلولهای سرطانی شده است. این اثر با افزایش غلظت عصاره نسبت معکوس داشته است. گرچه این اثر از نظر آماری تفاوت معناداری نشان نداده است. (0/05</span></span></span></span><span lang="AR-SA" style="font-size:11.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><</span></span></span></span><span dir="LTR" style="font-size:11.0pt"><span calibri="" style="font-family:"><span style="color:black"><span style="font-weight:normal">P</span></span></span></span><span lang="FA" style="font-size:11.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal">)</span></span></span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black"><span style="font-weight:normal">. همچنین کاهش میزان زیست پذیری در سلولهای فیبروبلاست نرمال لثه در اثر استفاده از غلظتهای مختلف عصاره نیز مشاهده شد (0/05</span></span></span></span><span lang="AR-SA" style="font-size:11.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><</span></span></span></span><span dir="LTR" style="font-size:11.0pt"><span calibri="" style="font-family:"><span style="color:black"><span style="font-weight:normal">P</span></span></span></span><span lang="FA" style="font-size:11.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal">)</span></span></span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black"><span style="font-weight:normal">. آزمون </span></span></span></span><span dir="LTR" style="font-size:11.0pt"><span new="" roman="" style="font-family:" times=""><span style="font-weight:normal">two way Anova</span></span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black"><span style="font-weight:normal"> نشان داد&nbsp; </span></span></span></span><span dir="LTR" style="font-size:11.0pt"><span new="" roman="" style="font-family:" times=""><span style="font-weight:normal">Interaction</span></span></span><span style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black"><span style="font-weight:normal"> یا اثر متقابل بین متغیر مستقل (غلظت عصاره) و متغیر وابسته (زیست پذیری سلولها) بسته به متغیر مستقل (نوع سلول سرطانی و یا سالم) معنا دار نیست. (0/8</span></span></span></span><span lang="AR-SA" style="font-size:11.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><</span></span></span></span><span dir="LTR" style="font-size:11.0pt"><span calibri="" style="font-family:"><span style="color:black"><span style="font-weight:normal">P</span></span></span></span><span lang="FA" style="font-size:11.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal">)</span></span></span></span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black"><span style="font-weight:normal"></span></span></span></span></span></span></span></span></span></span></h3> <h3 dir="RTL" style="text-align:justify"><span style="font-size:8pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span bold="" new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">نتیجه گیری:</span></span></span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black"><span style="font-weight:normal">به نظر می رسد عصاره گل محمدی موجب کاهش زیست پذیری سلول های سرطانی و سلول های فییروبلاست نرمال لثه می گردد.</span></span></span></span></span></span></span></span></span></span></h3> <span style="font-size:12pt"><span new="" roman="" style="font-family:" times=""><b>Background and Aim:</b> <span style="font-size:11.0pt">Conventional cancer treatments usually have complex complications. Also, the resistance of cancer cells to treatments is a special point that indicates the need for alternative therapies such as the use of herbal compounds. The aim of this study was to evaluate the anticancer effect of aqueous extract of Persia rose on oral cancer cell line.</span></span></span><br> <span style="font-size:12pt"><span new="" roman="" style="font-family:" times=""><b>Material and Methods:</b> <span lang="EN" style="font-size:11.0pt">In this experimental study, the toxicity effect of aqueous extract of Persia rose with different concentrations over 48 hours and repeated 5 times on the viability of human oral carcinoma cells (NCBI cell line No # C152) and Normal gingival fibroblast cells (HF2FF cell line) were examined by MTT method and finally, the optical density of the solution obtained at 570 nm was read by Elisa Reader. The results were evaluated using Two Way ANOVA test and Tukey POST HOC test was used to compare concentrations.</span></span></span><br> <span style="font-size:12pt"><span new="" roman="" style="font-family:" times=""><b>Results:</b> <span style="font-size:11.0pt">The results showed that the aqueous extract of Persia rose had a negative effect on the viability of cancer cell line compared to the control group and reduced the viability of cancer cells. This effect was inversely related to increasing the extract concentration. However, this effect did not show a statistically significant difference (p> 0.05). Decreased viability in normal gingival fibroblasts was also observed due to the use of different concentrations of the extract (p> 0.05). Two-way ANOVA test showed that the interaction between the independent variable (extract concentration) and the dependent variable (cell viability) was not significant depending on the independent variable (cancer cell type or healthy) (p> 0.8).</span></span></span><br> <span style="font-size:12pt"><span new="" roman="" style="font-family:" times=""><b>Conclusion:</b> <span style="font-size:11.0pt">It seems Persia rose extract cause reduction of cancer cells and normal gingival fibroblasts viability.</span></span></span><br> &nbsp; رزاسه, سمیت, نئوپلاسم دهان  Rosaceae, toxicity, oral  neoplasm 185 193 http://jrds.ir/browse.php?a_code=A-10-1081-3&slc_lang=fa&sid=1 Hesaneh Shakeri Mobasser حسانه شاکری مبصر shakeryhesane@gmail.com 100319475328460020350 100319475328460020350 No دندانپزشک عمومی Sareh Farhadi ساره فرهادی dr.sfarhadi@gmail.com 100319475328460020351 100319475328460020351 No گروه آسیب شناسی دهان، فک و صورت، دانشکده دندانپزشکی دانشگاه علوم پزشکی آزاد اسلامی، تهران، ایران Maryam Jolehar مریم جولهر joleharm@gmail.com 100319475328460020352 100319475328460020352 Yes گروه آسیب شناسی دهان، فک و صورت، دانشکده دندانپزشکی دانشگاه علوم پزشکی آزاد اسلامی، تهران، ایران