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:: Volume 15, Issue 4 (JRDS 2019) ::
J Res Dent Sci 2019, 15(4): 211-217 Back to browse issues page
Evaluation of effect of four types of fissure sealant on growth inhibition of streptococcusmutans and lactobacillus acidophilus )In vitro(
M Moradi dehnavi , N Amini * , N Askarizadeh , J Vandyousefi , A Tolouei
Abstract:   (5651 Views)
Abstract
Background and Aim: Since some sealants can  control cariogenic microorganisms, therefore the evaluation of antibacterial  effect of different sealants are the subject of this study.
Materials and Methods: in this experimental study,  the plates were divided into 5 groups.Group I control:two plates including Blood Agar with Mutans Streptococcus (SM) & the other MRS with Lactobacillus Acidophilus (LA).Group II:6 plates Blood Agar culture with two wells and inoculation of SM with addition of two fissure sealants; Clinpro 3M with fluoride &flowable composite 3M without fluoride.In group III all the steps were the same as group IIexcept that in which fissure sealants used were  Conseal F with flouride& Eco-s without fluoride.In groups IV & V all the steps were the same as groups II & III respectively except that MRS culture media with inoculation of Lactobacillus Acidophilus was used.The diameter of the growth inhibition halo (zone of inhibition) was measured using calipers. The experiment was repeated for three consecutive days with 26 plates  and the results were recorded every 48 hours.The data were analyzed using Two-way ANOVA
Results: The diameter of growth inhibition halo for Clinpro 3M in the medium where Streptococcus mutans was cultured was 27/29+ 3/59mm&where Lactobacillus Acidophilus was cultured was 12/37+ 1/46mm which differed from other three materials significantly. (p<0.000)
Conclution: It seems, Clinpro 3M had the most inhibitory effect on Streptococcus mutans and , Lactobacillus acidophilus  growth.
 
Keywords: Antibacterial agents, Fissure Sealants, Streptococcus mutans, Lactobacillus, Clinpro sealant.
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Type of Study: original article | Subject: Pediatrics
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Moradi dehnavi M, amini N, askarizadeh N, vandyousefi J, Tolouei A. Evaluation of effect of four types of fissure sealant on growth inhibition of streptococcusmutans and lactobacillus acidophilus )In vitro(. J Res Dent Sci 2019; 15 (4) :211-217
URL: http://jrds.ir/article-1-984-en.html


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Volume 15, Issue 4 (JRDS 2019) Back to browse issues page
مجله تحقیق در علوم دندانپزشکی Res Dent Sci
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